Signalling

Part:BBa_K2543010:Design

Designed by: CHUNG-HSUAN HSIAO   Group: iGEM18_Mingdao   (2018-10-08)


Ac5-hCD4-dToll-polyA / pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal XhoI site found at 3691
    Illegal XhoI site found at 4153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3760
    Illegal NgoMIV site found at 5079
    Illegal AgeI site found at 3742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2923
    Illegal BsaI site found at 4549
    Illegal BsaI.rc site found at 2533


Design Notes

CD4 fused dToll with AgeI and NgoMIV following the RFC[25] assembly rule


Source

The part was assembled with the existing parts


References